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. 2017 Jan 30;8:105. doi: 10.3389/fmicb.2017.00105

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Copyright © 2017 Yeware, Shurpali, Athalye and Sarkar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of superoxide produced by M. smegmatis cells. (A) Cell concentration-dependent production of superoxide: Different concentrations of M. smegmatis cells were incubated with DHE for 90 min. After incubation, samples were further processed for HPLC analysis. Levels of 2-hydroxyethdium (2-EOH; dark bar) and ethidium (Et; gray bar) were determined from the standard plot.. The results represent the mean ± SD of three identical experiments. (p < 0.05). (B) Time-dependent increase in superoxide production in (i) M. smegmatis and (ii) M. smegmatis membrane preparation: Approximately 2.2 × 10⁸ cells/mL and 15 μg of the membrane protein were distinctly incubated with DHE at different time points. Samples were further processed for HPLC analysis. Levels of 2-EOH (•) and Et (▲) were determined from the standard plot. The results represent the mean ± SD of three identical experiments. (p < 0.05).