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Effect of different ROS modulators on superoxide production in M. smegmatis. Rotenone (10 μM), antimycin A (10 μM), DPI (15 μM), tempol (10 μM), and PEG-SOD (5 units/mL) were incubated with (A) M. smegmatis and (B) membrane preparation. Samples were further processed for HPLC analysis. The results are expressed as fold change measured as the ratio of treated to untreated samples (control). The results represent the mean ± SD of three identical experiments. (∗p < 0.05; ∗∗p < 0.1).
