FIGURE 6.

Involvement of superoxide in the growth of M. smegmatis. (A) Effect of ROS modulators and generators on superoxide production by M. smegmatis. M. smegmatis cells were incubated with DPI (15 μM), DPI (15 μM) + menadione (150 μM), DPI (15 μM) + pyrogallol (300 μM), menadione (150 μM), and pyrogallol (300 μM) for 90 min. Samples were further processed as described in Figure 3. The results represent the mean ± SD of three identical experiments. (p < 0.05). (B) Effect of ROS modulators and generators on the growth of M. smegmatis. Log phase culture of M. smegmatis consisting of ∼2.3 × 10⁸ cells/mL was incubated with DPI (15 μM), DPI (15 μM) + menadione (150 μM), DPI (15 μM) + pyrogallol (300 μM), menadione (150 μM), and pyrogallol (300 μM) for 24 h, after which plating was done on Dubos agar plates. CFU measurements were performed after 3 days of incubation. Data shown are representative of three independent experiments.