Figure 7.

Overexpression of miR-34b-5p suppresses the MDA5 signaling pathway and promotes ALV-J replication. The (A) mRNA and (B) protein expression level of env were upregulated in DF-1 that overexpressed miR-34b-5p cells 3 days after the ALV-J infections. (C) The expression of mRNA of MDA5 signaling pathway-related innate and antiviral genes was downregulated in DF-1 cells that overexpressed miR-34b-5p. (D) The sample-to-positive ratio of virion secreted in supernatants was upregulated in DF-1 cells that overexpressed miR-34b-5p (n = 6). In panels (A–C), the values are expressed as the mean ± the SEM of three independent experiments. In panel (D), the results were confirmed by three independent experiments with six (cell number) samples per treatment. Asterisks denote statistically significant differences: ^* and ^** indicate P < 0.05 and P < 0.01, respectively. ALV-J, Avian leukosis virus subgroup J; MDA5, melanoma differentiation-associated gene 5; SEM, standard error of the mean.