FIGURE 3.

Sir2p agonists and antagonist alter virulence of H99 in Galleria. (A) INAM and resveratrol given to waxworms infected with 2 × 10⁴ H99 cells resulted in their prolonged survival, whereas sirtinol treatment resulted in decreased survival. (B) Lifespan effects were dependent on Sir2p and not observed when infections were done with sir2Δ cells. (C) SirAct and SRT1460 given to waxworms infected with 2 × 10⁴ H99 cells resulted in their prolonged survival. (D) Lifespan effects were dependent on Sir2p and not observed when infections were done with sir2Δ cells. Virulence experiments with the respective drug (n = 20–40 worms) represent duplicate experiments and were performed with the respective mutant at the same time. p-values were calculated by Log-Rank test.