Table 1.
Characterization of sir2Δ phenotypes in Cryptococcus neoformans strain H99.
| Phenotype | Phenotype in the mutant compared to the wt | Supporting information |
|---|---|---|
| Doubling time in YPD | Longer | 3.0 vs. 1.9 h (p < 0.01) |
| Doubling time in 0.05% YPD | No measured difference | 3.9 vs. 3.2 h |
| Mating | Sterile | No mating with Kn99MATa |
| Chronological lifespan | Decreased | 20 vs. 24 days |
| Phenotypic switching rate | No switching in either mutant or wt | n/a |
| Capsule size | Decreased | 0.84 + 0.2 vs. 0.96 + 0.2 μm (p < 0.01) |
| Total cell size | No measured difference | 6.48 + 0.8 vs. 6.75 + 0.7 μm |
| Phagocytosis in murine macrophages | No measured difference | 81.7 vs. 83.5% |
| Killing in murine macrophages | No measured difference | 76.7 vs. 80.5% |
| Colony sectoring | No sectoring in either mutant or wt | n/a |
| GXM staining | Same pattern | Punctate to 18B7 mAb |
| MIC to amphotericin B | No measured difference | 0.25 vs. 0.25 μg/ml |
| H2O2 resistance | No measured difference | 3.6 vs. 3.5 cm |
Results not significant unless stated otherwise.