Table 1.
Heterologous expression of pmiA genes in PseuM-susceptible strains.
| Indicator strain | PmiA immunity protein | PseuM bacteriocin | |||
|---|---|---|---|---|---|
| Species | Gene product | PaeM P. aeruginosa NCTC10332 | PflM P. fluorescens Q8r1-96 | PsyM P. syringae pv. tomato DC3000 | |
| P. aeruginosa CPHL12447 | P. aeruginosa | PmiANCTC10332 | − | ||
| PmiA*NCTC10332 | − | ||||
| P. fluorescens | PmiAQ8r1-96 | + | |||
| P. syringae pv. tomato | PmiADC3000 | + | |||
| NA | Control | + | |||
| P. fluorescens F113 | P. aeruginosa | PmiANCTC10332 | + | ||
| P. brassicacearum | PmiADF41 | − | |||
| P. fluorescens | PmiAQ8r1-96 | − | |||
| PmiA*Q8r1-96 | − | ||||
| PmiAAU2390 | + | ||||
| PmiAPf29Arp | − | ||||
| P. syringae pv. syringae | PmiADC3000 | + | |||
| Pseudomonas sp. | PmiA5 | T | |||
| PmiAGM21 | + | ||||
| PmiAPf153 | − | ||||
| NA | Control | + | |||
| P. syringae LMG 5456 | P. aeruginosa | PmiANCTC10332 | + | ||
| P. syringae pv. tomato | PmiADC3000 | − | |||
| PmiA*DC3000 | − | ||||
| NA | Control | + | |||
Indicator strains were transformed with shuttle vector pJB3Tc20 (control, without insert) or with pJB3Tc20-derived plasmids equipped with different pmiA genes, and tested for (altered) susceptibility to their respective PseuM via bacteriocin spot assay. +, sensitive; −, insensitive; T, turbid halo. The species used as source of immunity protein are specified with the respective strain names indicated as PmiA subscript (NA, not applicable). Asterisks define mutated immunity proteins (Asp to Ala). Strain LMG 5456 carrying a plasmid equipped with pmiAQ8r1−96 could not be obtained.