TABLE 1.
Growth characteristics of Bacteroides fragilis and other bacteria in BFS medium
| Isolate type and organism (no. of isolates)a | Colony profile on BFS agar (no. of isolates) |
|||||
|---|---|---|---|---|---|---|
| Esculin positivef | Growth after 48 h |
Yellow colonies | Blackening of the medium | |||
| Absent | Good | Poorg | ||||
| Anaerobic bacteria (215) | ||||||
| Bacteroides caccae | 4 (4) | 4 | 4 | 4 | ||
| Bacteroides dorei | 4 (4) | 4 | 4 | 4 | ||
| Bacteroides eggerthii | 1 (1) | 1 | 1 | 1 | ||
| Bacteroides fragilis (division I) | 52 (52) | 52 | 52 | 52 | ||
| Bacteroides fragilis (division II) | 52 (52) | 52 | 52 | 52 | ||
| Bacteroides intestinalis | 2 (2) | 2 | 2 | 2 | ||
| Bacteroides massiliensis | 1 (0) | 1 | 1 | |||
| Bacteroides nordii | 4 (4) | 4 | 4 | 4 | ||
| Bacteroides ovatus | 11 (11) | 11 | 11 | 11 | ||
| Bacteroides pyogenes | 2 (0) | 2 | 2 | |||
| Bacteroides salyersiae | 2 (2) | 2 | 2 | 2 | ||
| Bacteroides stercoris | 2 (2) | 2 | 2 | 2 | ||
| Bacteroides thetaiotaomicron | 10 (10) | 10 | 10 | 10 | ||
| Bacteroides uniformis | 10 (10) | 10 | 10 | 10 | ||
| Bacteroides vulgatus | 10 (4) | 10 | 10 | 4 | ||
| Clostridium species | 15 (3) | 15 | ||||
| Fusobacterium speciesb | 3 (0) | 2 | 1 | 1 | ||
| Parabacteroides distasonis | 2 (2) | 2 | 2 | 2 | ||
| Parabacteroides faecis | 2 (0) | 2 | 2 | |||
| Parabacteroides goldsteinii | 2 (0) | 2 | 2 | |||
| Peptostreptococcus species | 3 (0) | 3 | ||||
| Prevotella species | 16 (1) | 16 | ||||
| Other anaerobesc | 5 (0) | 5 | ||||
| Aerobic and facultative anaerobic bacteria (88) | ||||||
| Enterobacteriaceae | 16 (8) | 16 | ||||
| Enterococcus species | 6 (6) | 6 | ||||
| Lactobacillus species | 5 (5) | 5 | ||||
| Nonfermentersd | 7 (2) | 7 | ||||
| Staphylococcus species | 23 (0) | 23 | ||||
| Streptococcus species | 22 (4) | 22 | ||||
| Vibrio species | 6 (2) | 6 | ||||
| Other bacteriae | 3 (2) | 3 | ||||
| Total | 303 (193) | 129 | 138 | 36 | 174 | 160 |
The total includes 48 reference strains and 255 clinical isolates. The designations of the reference strains and the clinical isolates are listed in Tables S1 and S2 in the supplemental material.
Fusobacterium mortiferum (n = 1; good growth), F. varium (n = 1, absent), and F. nucleatum (n = 1, absent).
Bifidobacterium bifidum (n = 1), Finegoldia magna (n = 1), Parvimonas micra (n = 1), Propionibacterium acnes (n = 1), and Veillonella parvula (n = 1).
Acinetobacter baumannii (n = 2), Brevundimonas diminuta (n = 1), Flavobacterium meningosepticum (n = 1), Pseudomonas aeruginosa (n = 2), and Stenotrophomonas maltophilia (n = 1).
Aeromonas hydrophila (n = 1), Kocuria rosea (n = 1), and Listeria monocytogenes (n = 1).
Ability of the organisms to hydrolyze esculin was determined by separate testing.
Poor growth is defined by pinpoint-sized colonies after 48 h of incubation.