TABLE 2.
Evaluation of the BYG Carba test, β Carba test, Rapidec Carba NP test, and Neo-Rapid Carb screen kit against 130 isolates of nonfermenting Gram-negative bacteria
| Genus | Carbapenemase | No. of isolates | MIC range (μg/ml) for: |
Test results (no.) fora: |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Meropenem | Imipenem | BYG |
BCT |
RCNP |
NRCK |
||||||||||||
| P | N | P | D | N | P | D | N | NI | P | D | N | NI | |||||
| Pseudomonas spp. | GESb | 4 | >32 | 16–>32 | 1 | 3 | 0 | 0 | 4 | 2 | 0 | 2 | 0 | 2 | 0 | 2 | 0 |
| KPC-2 | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| VIMc | 34 | 32–>32 | 32–>32 | 32 | 2 | 34 | 0 | 0 | 32 | 0 | 2 | 0 | 33 | 0 | 0 | 1 | |
| IMPd | 11 | 24–>32 | >32 | 10 | 1 | 11 | 0 | 0 | 11 | 0 | 0 | 0 | 11 | 0 | 0 | 0 | |
| NDM-1 | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
| GIM | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| DIM | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| SPM-1 | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| OXA-198 | 1 | 12 | >32 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | |
| Total carbapenemase producers | 55 | 12–>32 | 16–>32 | 48 | 7 | 50 | 1 | 4 | 50 | 0 | 5 | 0 | 51 | 0 | 2 | 2 | |
| Non-carbapenemase producerse | 34 | 0.38–>32 | 0.75–>32 | 2 | 32 | 2 | 0 | 32 | 1 | 2 | 30 | 1 | 0 | 1 | 30 | 3 | |
| Acinetobacter spp. | GES-14 | 1 | 32 | 32 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
| VIM-4 | 1 | 32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| IMPf | 3 | 16–>32 | 3–>32 | 3 | 0 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | |
| NDM-1 | 2 | >32 | >32 | 2 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | |
| GIM | 1 | 8 | 4 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| OXA carbapenemasesg | 22 | 2–>32 | 4–>32 | 15 | 7 | 16 | 3 | 3 | 2 | 3 | 17 | 0 | 2 | 1 | 18 | 1 | |
| NDM-1 + OXA-23 | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |
| NDM-2 + OXA-23 | 1 | >32 | >32 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
| VIM-4 + OXA-58 | 1 | 32 | >32 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |
| Total carbapenemase producers | 33 | 2–>32 | 3–>32 | 24 | 9 | 25 | 4 | 4 | 12 | 3 | 18 | 0 | 9 | 2 | 21 | 1 | |
| Non-carbapenemase producersh | 8 | 0.25–32 | 0.19–8 | 0 | 8 | 1 | 0 | 7 | 0 | 2 | 6 | 0 | 0 | 0 | 8 | 0 | |
BYG, BYG Carba test, BCT, β Carba test, RCNP, Rapidec Carba NP test, NRCK, Neo-Rapid CARB screen kit; P, positive; N, negative; D, doubtful; NI, noninterpretable.
GES-2 (n = 1), GES-5 (n = 1), GES-18 (n = 2).
VIM-1 (n = 2), VIM-2 (n = 24), VIM-4 (n = 7), VIM-5 (n = 1).
Including IMP-1 (n = 1), IMP-2 (n = 1), IMP-7 (n = 1), IMP-13 (n = 5), IMP-15 (n = 1), IMP-19 (n = 1), and IMP-29 (n = 1).
Including strains producing BEL-1 (n = 2), BEL-2 (n = 1), GES-1 (n = 2), OXA-2 (n = 1), OXA-2a (n = 1), OXA-4 (n = 1), OXA-9 (n = 1), OXA-10 (n = 1), OXA-18 (n = 1), OXA-28 (n = 1), OXA-35 (n = 1), PME-1 (n = 1), SHV-2a (n = 1), SHV-5 (n = 1), TEM-4 (n = 1), combination of OXA-10 + TEM-1 (n = 1), and phenotypically assessed impermeability alone (n = 16).
Including IMP-1 (n = 1), IMP-4 (n = 1), and IMP-14 (n = 1).
Including OXA-23 (n = 10), OXA-24 (n = 1), OXA-25 (n = 1), OXA-26 (n = 1), OXA-27 (n = 1), OXA-58 (n = 4), OXA-72 (n = 1), OXA-143 (n = 1), OXA-255 (n = 1), and combination of OXA-23 with OXA-58 (n = 1).
Including strains producing CARB-5 (n = 1), CARB-10 (n = 1), GES-12 (n = 1), PER-1 (n = 1), VEB-1 (n = 1), phenotypically assessed impermeability (n = 1), and wild type (n = 2).