FIG 1.

Selected NleD transposon mutants that display altered proteolytic activity toward JNK and/or p38. (A) Immunoblots of JNK and p38 cleavage in cells transiently transfected with GFP-NleD or GFP-NleD mutant derivatives. Cell lysates were probed with anti-JNK and anti-p38 antibodies. Arrows, cleavage products. Anti-GFP antibody was used to show the expression of GFP-NleD and GFP-NleD variants. Actin was used as a loading control. The immunoblots are representative of those from at least 3 independent experiments. UT, untransfected. (B) Schematic diagram mapping transposon insertion sites in NleD that alter JNK and/or p38 cleavage. Dark gray arrowheads, little or no cleavage of JNK or p38; light gray arrowheads, cleavage of JNK but little or no cleavage of p38; white arrowheads, total cleavage of both JNK and p38. aa, amino acids.