FIG 3.

Analysis of NleD single site-directed mutants for p38 cleavage. Immunoblots of JNK and p38 cleavage in HEK293T cells transiently transfected with GFP-NleD or GFP-NleD single site-directed mutants are shown. Cell lysates were probed with anti-JNK and anti-p38 antibodies. Anti-GFP antibody was used to show the expression of GFP-NleD and GFP-NleD variants. Cleavage products are indicated by arrows. Actin was used as a loading control. The immunoblots are representative of those from at least 3 independent experiments. UT, untransfected.