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Alignment of zinc metalloprotease motif and the surrounding area in NleD proteins from various Gram-negative bacteria. Sequences were identified through BLAST analysis using NleD from EPEC O127:H6 strain E2348/69 as a reference. A section of the proteins identified by BLAST analysis was aligned using the Clustal Omega program, and presentation of the alignment was performed using the ESPript (v3) program (36). *, HEXXH zinc metalloprotease site; arrow, conserved residue R203.
