TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pEGFP-C2 | Expression vector carrying enhanced GFP, Kanr | Clontech |
| pGFP-NleD | Full-length nleD from EPEC E2348/69 in pEGFP-C2, Kanr | 2 |
| pGFP-NleDG110A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid G110 mutated to A, Kanr | This study |
| pGFP-NleDT111A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid T111 mutated to A, Kanr | This study |
| pGFP-NleDF115A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid F115 mutated to A, Kanr | This study |
| pGFP-NleDE143A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid E143 mutated to A, Kanr | This study |
| pGFP-NleDL144A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid L144 mutated to A, Kanr | This study |
| pGFP-NleDH146A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid H146 mutated to A, Kanr | This study |
| pGFP-NleDN150A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid N150 mutated to A, Kanr | This study |
| pGFP-NleDE154A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid E154 mutated to A, Kanr | This study |
| pGFP-NleDR155A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid R155 mutated to A, Kanr | This study |
| pGFP-NleDV179A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid V179 mutated to A, Kanr | This study |
| pGFP-NleDG180A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid G180 mutated to A, Kanr | This study |
| pGFP-NleDR202A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid R202 mutated to A, Kanr | This study |
| pGFP-NleDR203A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid R203 mutated to A, Kanr | This study |
| pGFP-NleDR203E | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid R203 mutated to E, Kanr | This study |
| pGFP-NleDR203K | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid R203 mutated to K, Kanr | This study |
| pGFP-NleDT204A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid T204 mutated to A, Kanr | This study |
| pGFP-NleDN217A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid N217 mutated to A, Kanr | This study |
| pGFP-NleDT218A | nleD from EPEC E2348/69 in pEGFP-C2 with amino acid T218 mutated to A, Kanr | This study |
| pGFP-NleDLDK(59–61)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the LDK(59–61) motif mutated to AAA, Kanr | This study |
| pGFP-NleDLN(72–73)AA | nleD from EPEC E2348/69 in pEGFP-C2 with the LN(72–73) motif mutated to AA, Kanr | This study |
| pGFP-NleDIES(75–77)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the IES(75–77) motif mutated to AAA, Kanr | This study |
| pGFP-NleDHR(100–101)AA | nleD from EPEC E2348/69 in pEGFP-C2 with the HR(100–101) motif mutated to AA, Kanr | This study |
| pGFP-NleDRGT(109–111)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the RGT(109–111) motif mutated to AAA, Kanr | This study |
| pGFP-NleDVF(147–148)AA | nleD from EPEC E2348/69 in pEGFP-C2 with the VF(147–148) motif mutated to AA, Kanr | This study |
| pGFP-NleDDFH(114–116)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the DFH(114–116) motif mutated to AAA, Kanr | This study |
| pGFP-NleDHVF(146–148)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the HVF(146–148) motif mutated to AAA, Kanr | This study |
| pGFP-NleDHNL(149–151)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the HNL(149–151) motif mutated to AAA, Kanr | This study |
| pGFP-NleDGER(153–155)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the GER(153–155) motif mutated to AAA, Kanr | This study |
| pGFP-NleDTVG(178–180)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the TVG(178–180) motif mutated to AAA, Kanr | This study |
| pGFP-NleDREE(195–197)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the REE(195–197) motif mutated to AAA, Kanr | This study |
| pGFP-NleDRRT(202–204)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the RRT(202–204) motif mutated to AAA, Kanr | This study |
| pGFP-NleDDNT(216–218)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the DNT(216–218) motif mutated to AAA, Kanr | This study |
| pGFP-NleDQVR(225–227)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the QVR(225–227) motif mutated to AAA, Kanr | This study |
| pGFP-NleDRLH(227–229)AAA | nleD from EPEC E2348/69 in pEGFP-C2 with the RLH(227–229) motif mutated to AAA, Kanr | This study |
| pET28a | N-terminal 6× His Tag cloning/expression vector | Novagen |
| pET-NleD | nleD from EPEC E2348/69 in pET28a, Kanr | This study |
| pET-NleDE143A | nleD from EPEC E2348/69 in pET28a with amino acid E143 mutated to A, Kanr | This study |
| pET-NleDR203E | nleD from EPEC E2348/69 in pET28a with amino acid R203 mutated to E, Kanr | This study |
| pGEX-4T-1 | N-terminal GST cloning/expression vector, Ampr | GE Healthcare |
| pGEX-p38 | Human p38 (MAPK14 transcript variant 2) in pGEX-4T-1, Ampr | This study |
| pTrc99A | Bacterial expression vector containing a lacI promoter, Ampr | Pharmacia Biotech |
| pNleD | nleD from EPEC 2348/69 in pTrc99A, Ampr | 12 |
| pNleDE143A | nleD from EPEC E2348/69 in pTrc99A with amino acid E143 mutated to A, Ampr | This study |
| pNleDR203E | nleD from EPEC E2348/69 in pTrc99A with amino acid R203 mutated to E, Ampr | This study |
| pNleD-2HA | nleD from EPEC 2348/69 in pTrc99A with a 2× C-terminal HA tag, Ampr | This study |
| pNleDE143A-2HA | nleD from EPEC E2348/69 in pTrc99A with amino acid E143 mutated to A and a 2× C-terminal HA tag, Ampr | This study |
| pNleDR203E-2HA | nleD from EPEC E2348/69 in pTrc99A with amino acid R203 mutated to E and a 2× C-terminal HA tag, Ampr | This study |