TABLE 3.
Primers used in this study
| Primer | Sequencea |
|---|---|
| Pos. 18F | GTCATCAACGTCGAGCTTGCGGCCGCAGAGCTCAATGTCAGATACAGATATCG |
| Pos. 18R | CGATATCTGTATCTGACATTGAGCTCTGCGGCCGCAAGCTCGACGTTGATGAC |
| Pos. 50F | CGTTTCCAGGGGACTGATCATGCGGCCGCAGATCATAATATATATCAGCAGATTG |
| Pos. 50R | CAATCTGCTGATATATATTATGATCTGCGGCCGCATGATCAGTCCCCTGGAAACG |
| Pos. 60F | GCAGATTGAAGCAGCACTCTGCGGCCGCACACTCGATAAGATTGGCTCTACAG |
| Pos. 60R | CTGTAGAGCCAATCTTATCGAGTGTGCGGCCGCAGAGTGCTGCTTCAATCTGC |
| Pos. 91F | CAGTGGTAATACACCTTGCGGCCGCACACCTCAACTCTTCCAGACTAGG |
| Pos. 91R | CCTAGTCTGGAAGAGTTGAGGTGTGCGGCCGCAAGGTGTATTACCACTG |
| Pos. 121F | CGATTTTCACTGTAATCTGAATTGCGGCCGCATGAATGCAGTTGAATATCCCTGTGG |
| Pos. 121R | CCACAGGGATATTCAACTGCATTCATGCGGCCGCAATTCAGATTACAGTGAAAATCG |
| Pos. 128F | CAGTTGAATATCCCTGTGGTGCGGCCGCATGTGGGGAGGGGATTAGCGTGGTG |
| Pos. 128R | CACCACGCTAATCCCCTCCCCACATGCGGCCGCACCACAGGGATATTCAACTG |
| Pos. 133F | GTGGGGAGGGGATTAGCGTTGCGGCCGCAAGCGTGGTGGACTTTCATGCGAC |
| Pos. 133R | GTCGCATGAAAGTCCACCACGCTTGCGGCCGCAACGCTAATCCCCTCCCCAC |
| Pos. 144F | CGACTATTGTTTTTCATGAGTGCGGCCGCAATGAGTTGCTCCATGTTTTCCAC |
| Pos. 144R | GTGGAAAACATGGAGCAACTCATTGCGGCCGCACTCATGAAAAACAATAGTCG |
| Pos. 167F | CCGACCAGAATCACAAAATGCGGCCGCACAAAAATACTCTCCACTTTTACTCG |
| Pos. 167R | CGAGTAAAAGTGGAGAGTATTTTTGTGCGGCCGCATTTTGTGATTCTGGTCGG |
| Pos. 191F | CTTTTTCAGAGGAGGTGCTTTCTGCGGCCGCACTTTCAGAAAATAAATTCCGCGAAGAG |
| Pos. 191R | CTCTTCGCGGAATTTATTTTCTGAAAGTGCGGCCGCAGAAAGCACCTCCTCTGAAAAAG |
| Pos. 207F | GATGCCCCGTAGAACCTCCTATGCGGCCGCATCCTACCCGCACGACTCAGCTCTTATTC |
| Pos. 207R | GAATAAGAGCTGAGTCGTGCGGGTAGGATGCGGCCGCATAGGAGGTTCTACGGGGCATC |
| LDK59-61AAAF | CAGCAGATTGAAGCAGCAGCGGCGGCGATTGGCTCTACAGAGACAGGG |
| LDK59-61AAAR | CCCTGTCTCTGTAGAGCCAATCGCCGCCGCTGCTGCTTCAATCTGCTG |
| LN72-73AAF | CAGAGACAGGGCGTGTACTCGCGGCGGCTATTGAATCAATATCC |
| LN72-73AAR | GGATATTGATTCAATAGCCGCCGCGAGTACACGCCCTGTCTCTG |
| IES75-77AAAF | CGTGTACTCCTGAATGCTGCGGCGGCGATATCCCGACTTAAATCAGAAACAGTG |
| IES75-77AAAR | CACTGTTTCTGATTTAAGTCGGGATATCGCCGCCGCAGCATTCAGGAGTACACG |
| HR100-101AAF | CCAGACTAGGAGTTATGGCAGCGGCGGATATAGATGCTGAGAACCATCG |
| HR100-101AAR | CGATGGTTCTCAGCATCTATATCCGCCGCTGCCATAACTCCTAGTCTGG |
| DFH114-116AAAF | CCATCGGGGGACTGGTTCCGCGGCGGCGTGTAATCTGAATGCAGTTGAATATCCC |
| DFH114-116AAAR | GGGATATTCAACTGCATTCAGATTACACGCCGCCGCGGAACCAGTCCCCCGATGG |
| HVF146-148AAAF | CGACTATTGTTTTTCATGAGTTGCTCGCGGCGGCGCACAATTTAAATGGGGAGCGTTTG |
| HVF146-148AAAR | CAAACGCTCCCCATTTAAATTGTGCGCCGCCGCGAGCAACTCATGAAAAACAATAGTCG |
| VF147-148AAF | GACTATTGTTTTTCATGAGTTGCTCCATGCGGCGCACAATTTAAATGGGGAGC |
| VF147-148AAR | GCTCCCCATTTAAATTGTGCGCCGCATGGAGCAACTCATGAAAAACAATAGTC |
| HNL149-151AAAF | CATGAGTTGCTCCATGTTTTCGCGGCGGCGAATGGGGAGCGTTTGAAAGTTGAGAGTTC |
| HNL149-151AAAR | GAACTCTCAACTTTCAAACGCTCCCCATTCGCCGCCGCGAAAACATGGAGCAACTCATG |
| GER153-155AAAF | CCATGTTTTCCACAATTTAAATGCGGCGGCGTTGAAAGTTGAGAGTTCCCGAC |
| GER153-155AAAR | GTCGGGAACTCTCAACTTTCAACGCCGCCGCATTTAAATTGTGGAAAACATGG |
| TVG178-180AAAF | CTCGAAGAAGCCAGGGCGGCGGCGTTGGGGGCTTTTTCAGAG |
| TVG178-180AAAR | CTCTGAAAAAGCCCCCAACGCCGCCGCCCTGGCTTCTTCGAG |
| REE195-197AAAF | GGTGCTTTCAGAAAATAAATTCGCGGCGGCGATTGGGATGCCCCGTAGAACC |
| REE195-197AAAR | GGTTCTACGGGGCATCCCAATCGCCGCCGCGAATTTATTTTCTGAAAGCACC |
| RRT202-204AAAF | CGAAGAGATTGGGATGCCCGCGGCGGCGTCCTACCCGCACGACTCAGC |
| RRT202-204AAAR | GCTGAGTCGTGCGGGTAGGACGCCGCCGCGGGCATCCCAATCTCTTCG |
| DNT216-218AAAF | GACTCAGCTCTTATTCATGATGCGGCGGCGGTGAGTCTGGGATTCCAACAGG |
| DNT216-218AAAR | CCTGTTGGAATCCCAGACTCACCGCCGCCGCATCATGAATAAGAGCTGAGTC |
| QVR225-227AAAF | CAGTGAGTCTGGGATTCCAAGCGGCGGCGCTGCATCCATTGCTTTAG |
| QVR225-227AAAR | CTAAAGCAATGGATGCAGCGCCGCCGCTTGGAATCCCAGACTCACTG |
| RLH227-229AAAF | GACAATACAGTGAGTCTGGGATTCCAACAGGTAGCGGCGGCGCCATTGCTTTAG |
| RLH227-229AAAR | CTAAAGCAATGGCGCCGCCGCTACCTGTTGGAATCCCAGACTCACTGTATTGTC |
| E143AF | GGACTTTCATGCGACTATTGTTTTTCATGCGTTGCTCCATGTTTTCCAC |
| E143AR | GTGGAAAACATGGAGCAACGCATGAAAAACAATAGTCGCATGAAAGTCC |
| G110AF | GATGCTGAGAACCATCGGGCGACTGGTTCCGATTTTCACTG |
| G110AR | CAGTGAAAATCGGAACCAGTCGCCCGATGGTTCTCAGCATC |
| T111AF | GCTGAGAACCATCGGGGGGCGGGTTCCGATTTTCACTG |
| T111AR | CAGTGAAAATCGGAACCCGCCCCCCGATGGTTCTCAGC |
| F115AF | CATCGGGGGACTGGTTCCGATGCGCACTGTAATCTGAATGCAG |
| F115AR | CTGCATTCAGATTACAGTGCGCATCGGAACCAGTCCCCCGATG |
| L144AF | GACTATTGTTTTTCATGAGGCGCTCCATGTTTTCCACAATTTAAATGG |
| L144AR | CCATTTAAATTGTGGAAAACATGGAGCGCCTCATGAAAAACAATAGTC |
| H146AF | CGACTATTGTTTTTCATGAGTTGCTCGCGGTTTTCCACAATTTAAATGG |
| H146AR | CCATTTAAATTGTGGAAAACCGCGAGCAACTCATGAAAAACAATAGTCG |
| N150AF | GTTGCTCCATGTTTTCCACGCGTTAAATGGGGAGCGTTTG |
| N150AR | CAAACGCTCCCCATTTAACGCGTGGAAAACATGGAGCAAC |
| E154AF | GTTTTCCACAATTTAAATGGGGCGCGTTTGAAAGTTGAGAG |
| E154AR | CTCTCAACTTTCAAACGCGCCCCATTTAAATTGTGGAAAAC |
| R155AF | CCACAATTTAAATGGGGAGGCGTTGAAAGTTGAGAGTTCCCGAC |
| R155AR | GTCGGGAACTCTCAACTTTCAACGCCTCCCCATTTAAATTGTGG |
| V179AF | CGAAGAAGCCAGGACTGCGGGGTTGGGGGCTTTTTC |
| V179AR | GAAAAAGCCCCCAACCCCGCAGTCCTGGCTTCTTCG |
| G180AF | CGAAGAAGCCAGGACTGTTGCGTTGGGGGCTTTTTCAGAGG |
| G180AR | CCTCTGAAAAAGCCCCCAACGCAACAGTCCTGGCTTCTTCG |
| R202AF | CGAAGAGATTGGGATGCCCGCGAGAACCTCCTACCCGCACG |
| R202AR | CGTGCGGGTAGGAGGTTCTCGCGGGCATCCCAATCTCTTCG |
| R203AF | GATTGGGATGCCCCGTGCGACCTCCTACCCGCAC |
| R203AR | GTGCGGGTAGGAGGTCGCACGGGGCATCCCAATC |
| R203EF | GATTGGGATGCCCCGTGAGACCTCCTACCCGCAC |
| R203ER | GTGCGGGTAGGAGGTCTCACGGGGCATCCCAATC |
| R203KF | GATTGGGATGCCCCGTAAGACCTCCTACCCGCAC |
| R203KR | GTGCGGGTAGGAGGTCTTACGGGGCATCCCAATC |
| T204AF | GAGATTGGGATGCCCCGTAGAGCGTCCTACCCGCACGACTCAG |
| T204AR | CTGAGTCGTGCGGGTAGGACGCTCTACGGGGCATCCCAATCTC |
| N217AF | GCTCTTATTCATGATGACGCGACAGTGAGTCTGGGATTCC |
| N217AR | GGAATCCCAGACTCACTGTCGCGTCATCATGAATAAGAGC |
| T218AF | CTCTTATTCATGATGACAATGCGGTGAGTCTGGGATTCCAACAG |
| T218AR | CTGTTGGAATCCCAGACTCACCGCATTGTCATCATGAATAAGAG |
| NleDF | AAGAATTCATGCGCCCTACGTCCCTC |
| NleDR | GGAGTCGACGCTAAAGCAATGGATGCAGTCTTAC |
| p38F | GGGGATCCATGTCTCAGGAGAGGCCCACG |
| p38R | CGCTCGAGTCAGGACTCCATCTCTTCTTGGTC |
| NleD-2HAF | AACCATGGATGCGCCCTACGTCCCTC |
| NleD-2HAR | CCGGATCCCTACGCATAATCCGGCACATCATACGGATACGCATAATCCGGCAC ATCATACGGATAAAGCAATGGATGCAGTCTTACC |
| pTrcF | AACACCCCCATCGGCG |
| pTrcR | GTAACCATTATAAGCTGC |
| pEGFP-C2F | AACACCCCCATCGGCG |
| pEGFP-C2R | GTAACCATTATAAGCTGC |
| NotI | TGCGGCCGCA |
a
Boldface nucleotides indicate mutated codons.