FIG 5.

Y. pseudotuberculosis infection does not influence the flagellin-specific gene expression signature. (A and B) Female C57BL/6 mice (n = 4) were treated intraperitoneally with flagellin (5 μg) in PBS or with PBS alone (mock). Thirty minutes later, the animals were challenged intragastrically with 5 × 10⁸ CFU of Y. pseudotuberculosis. One group of mice was treated with flagellin but not Y. pseudotuberculosis. Ilea were sampled postinfection and mRNA was quantified by quantitative reverse transcription-PCR. mRNA levels are expressed relative to those of uninfected and PBS-treated animals (the mock group; arbitrarily set to a value of 1) and are shown as the means ± SD. (A) Analysis of gene expression at 2 h posttreatment. Intergroup differences compared to flagellin-treated animals were analyzed in a Limma test with Benjamini-Hochberg FDR correction (*, P < 0.05; **, P < 0.01). Significant differences in mRNA expression levels were not found between flagellin and Y. pseudotuberculosis plus flagellin groups. (B) A time course analysis of gene expression.