FIG 7.

Flagellin protection does not require B, T, NK, and innate lymphoid cells. Rag2^−/− Il2rg^−/− mice were treated intraperitoneally with flagellin (5 μg) in PBS or with PBS alone 30 min before an intragastric challenge with 5 × 10⁸ CFU of Y. pseudotuberculosis. Bacterial counts were determined in the stools (A) and the spleen (B). CFU counts for individual mice (n = 13 to 16) at 72 h postinfection are shown. The solid bar corresponds to the median value, and the dashed line represents the detection threshold. Intergroup differences were analyzed in a Mann-Whitney test (**, P < 0.01).