FIG 5.

Alkalinization of the phagosome by C. albicans is required for macrophage killing and induction of pyroptotic cell death. C. albicans SC5314 and the ssy1Δ, dur1,2Δ, and ato5Δ mutants and their complemented strains were cocultured with J774A.1 macrophages for 5 h in a 96-well plate in the presence or absence of the caspase-1 inhibitor z-YVAD-fmk. Some of the wells were pretreated with 50 nM BafA for 30 min prior to coculture. The vATPase inhibitor was removed after 1 h of coculture, and all of the cultures were incubated for an additional 4 h. Macrophage lysis (A) and the release of IL-1β (B) were assessed as described in Materials and Methods. The experiments were performed in triplicate.