TABLE 2.
Bacterial strains, plasmids, and cell lines used in this study
| Strain, plasmid, or cell line | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus strains | ||
| RN6390 | 8325-4 wild type | 39 |
| QT7 | 8325-4 tet38::cat or Δtet38 | 40 |
| RN6390(pLZ113-tet38) | tet38 overexpressor | This study |
| RN6390(pAH16) | This study | |
| QT7(pAH16) | This study | |
| Plasmids | ||
| pAH14 | Plasmid for sarA P1-dependent YFP 10B expression, Ermr | 36 |
| pAH16 | pAH14 with sod ribosome binding site | 36 |
| pLZ113 | E. coli-S. aureus shuttle vector pRB373 with xyl/tet promoter cloned into the multiple cloning site | 41 |
| Cell lines | ||
| A549 | Human lung adenocarcinoma | 11 |
| Jurkat | Leukemic T-cell lymphoblast | 11 |