Figure 3.

The effect of apigenin exposure on the clonogenicity of cervical cancer cell lines and human keratinocytes at 1, 6, 18, 24, and 48 h and 7 and 14 days followed by culture with DMEM. The graph indicates that colony recovery diminished with increasing times of exposure in cervical cancer cell lines ((a) HeLa; (b) SiHa; (c) CaSki; (d) C33A) and increased with time of exposure in HaCaT cells (e). Data are shown as the mean values ± SD of three independent experiments conducted in triplicate. Photos indicate that exposure to apigenin reduced colony formation by 7 days and prevented colony formation after 14 days in HeLa (a), SiHa (b), CaSki (c), and C33A (d) cells. In HaCaT cells (f), colony formation continued to be equivalent after 7 and 14 days of apigenin exposure.