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. 2017 Jan 16;2017:8519169. doi: 10.1155/2017/8519169

Figure 6.

Figure 6

Autophagy triggered by HSYA-SDT through the PI3K/Akt/mTOR pathway and the inhibition of inflammatory factors were suppressed by the ROS scavenger NAC. (a) The relative fluorescence intensity of ROS generation detected in THP-1 macrophages with or without pretreatment with NAC was measured by flow cytometry. (b) The effect of NAC on the expression levels of mTOR, p-mTOR (Ser 2448), AKT, p-AKT (Ser 473), LC3-Ι, LC3-ΙΙ, p62, and beclin 1 at 30 min after SDT was determined, and quantifications of the proteins above are shown. (c) Protein extracts from untreated cells (Control) and cells treated with NAC, HSYA-SDT, or NAC prior to HSYA-SDT were analyzed by Western blots to detect TNF-α, IL-12, and IL-1β. Quantifications of protein expression are also provided. (d) ELISAs of the inflammatory factors TNF-α, IL-12, and IL-1β secreted by THP-1 macrophages with or without pretreatment by NAC. All data are mean ± standard error (n = 5). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus Control.