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. 2017 Jan 30;7:41165. doi: 10.1038/srep41165

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(a,b) Short-term growth curves of hPSCs in each culture manner. (a) H9 hESCs in TeSR-E8 medium and (b) H9 hESCs in StemFit AK03 medium. hPSCs cultured in the uncoated manner proliferated and had a similar growth rates as those in the precoated manner. (c) Phase-contrast images of H9 hESCs in TeSR-E8 and StemFit AK03 medium. Similar to the precoated manner, cells cultured in the uncoated manner proliferated normally without any colony detachment. Scale bar: 100 µm.