Figure 1. Sorting strategy and morphology of SPBs, BMBs, and BMMCs.

(a) YFP⁺ basophils (SPBs) from 6-week-old spleens of Mcpt8-cre-YFP mice were sorted and the purity was analyzed by flow cytometry (n = 3). (b) BM-derived c-kit^-CD49b⁺ basophils and c-kit⁺ CD49b⁺ MCs were isolated and the purity of each population was analyzed by flow cytometry. (c) Isolated SPBs, BMBs, and BMMCs were stimulated with IgE, LPS, or CT, and stained with Giemsa followed by analysis by light microscope (400x, scale bar = 10 μm). (d) 4 × 10⁶ cell lysates from BMB (Bs) for 1-week culture, BMMCs (MC) for 4 weeks culture, and BMDCs (DC) for 1-week culture were subjected to western blot analysis for MCP-11 protein expression. Full-length blots are presented in Supplementary Figure 1a. Data are representative of three independent experiments (n = 3).