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. 2017 Jan 30;7:41738. doi: 10.1038/srep41738

Figure 1. Mild hypothermia (32 °C) prevents SH-SY5Y neuroblastoma cells from NO-induced apoptosis.

Figure 1

SH-SY5Y cells were pre-cultured under normothermic (37 °C) or mild hypothermic (32 °C) conditions for 1 d and treated with (A) various concentrations of SNP at 37 °C for 16 h, or (B) treated with 0.5 mM SNP for 16–48 h, and cell viability was assessed by MTT assay. (C) 16 h post SNP (1 mM) treatment, cleaved (cl.) PARP was detected by Western blotting. β-actin served as a loading control. (D) 16 h post SNP (1 mM) treatment, cells were stained with DAPI (blue) to evaluate the effects of mild hypothermia on NO-induced apoptosis. *P < 0.05 and ***P < 0.001 versus control group. All results shown are representative of three independent experiments.