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. 2017 Jan 30;7:41738. doi: 10.1038/srep41738

Figure 2. RBM3 silencing by siRNA abolishes neuroprotective effect of mild hypothermia (32 °C).

Figure 2

(A) SH-SY5Y cells were pre-cultured at 37 °C or 32 °C for 1 d and the induction of RBM3 protein was detected by Western blotting. (B) 2 d post transfection of RBM3 siRNA or scramble siRNA (ctl), cells were continued to culture at 37 °C for 1–3 d, and MTT assay was performed to evaluate cytotoxicity of siRNAs. (C–E) 2 d post transfection of siRNAs, cells were pre-cultured for 1 d at 37 °C or 32 °C and then treated with SNP (1 mM) for 16 h. (C) Cell viability was assessed by MTT assay. (D) Cleaved PARP was detected by Western blotting. β-actin served as a loading control. (E) Histogram analysis for the relative levels of cleaved PARP in panel D. Results were normalized by β-actin. *P < 0.05 and ***P < 0.001 versus control group.