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. 2017 Jan 30;7:41738. doi: 10.1038/srep41738

Figure 4. RBM3 potently inhibits NO-induced p38 activation.

Figure 4

(A) SH-SY5Y cells transfected with plasmid pXJ40-myc (Veh) or pXJ40-myc-RBM3 (RBM3) were treated with SNP (1 mM) for 1–16 h, and Western blotting were performed to detect total and phosphorylated AMPKα, AKT, p38α, JNK1/2, and ERK1/2. (B) Western blotting was used to detect the phosphorylated MKK3/MKK6 at 16 h post SNP treatment. (C) Cells were pre-cultured at 37 °C or 32 °C for 1 d and treated with SNP for 16 h. Western blotting were performed to detect RBM3 and phosphorylated p38 (p-p38). (D) Histogram analysis for the relative levels of p-p38 in panel C. Results were normalized by total p38. ***P < 0.001 versus control group.