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. 2016 Nov 14;6(1):1–7. doi: 10.1242/bio.021519

Fig. 1.

Fig. 1.

Female Rev-erbα−/− mice display signs of reduced fertility. (A) Rev-erbα−/− (red bar) mating pairs produce significantly less pups per litter compared to Rev-erbα+/− (black bar) pairs. Unpaired two-tailed t-test, ***P<0.0001, n=18 for Rev-erbα+/− and n=8 for Rev-erbα−/−, F-test reveals no difference in variance, F=2.41, DFn=17, Dfd=7. (B) Number of litters per mating period is reduced in Rev-erbα−/− (red bar) mating pairs compared to Rev-erbα+/− (black bar) pairs. Unpaired two-tailed t-test, ***P<0.0001, n=13 for Rev-erbα+/− and n=8 for Rev-erbα−/−, F-test reveals difference in variance, F=51.38, DFn=12, Dfd=7. (C) Inter-litter period is significantly longer in Rev-erbα−/− (red bar) compared to Rev-erbα+/− (black bar) mating pairs. Unpaired two-tailed t-test, ***P<0.0001, n=16 for Rev-erbα+/− and n=6 for Rev-erbα−/−, F-test reveals difference in variance, F=8.793, DFn=5, Dfd=15. (D) Hepatic Fgf21 mRNA in the liver is increased in Rev-erbα−/− (red line) compared to Rev-erbα+/+ mice (black line). Two-way ANOVA with Bonferroni post-test. The two curves are significantly different, P=0.0008, n=4. (E) Plasma FGF21 protein levels are significantly increased in Rev-erbα−/− (red line) compared to Rev-erbα+/+ animals (black line). Two-way ANOVA with Bonferroni post-test. The two curves are significantly different, P=0.0003, n=4-6. All values are mean±s.e.m.