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. 2017 Jan 15;6(1):100–108. doi: 10.1242/bio.022111

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Analysis by flow cytometry of fibroblast (CD13) and pluripotency (SSEA-4 and TRA-1-60) markers during reprogramming of hADFs. (A) Percentages of CD13⁺, SSEA-4⁺ and TRA-1-60⁺ cells on different days of reprogramming in the TRA-1-85⁺ population of cells. (B,C) Percentages of the cells that co-express CD13 and SSEA-4 in the (B) TRA-1-85⁺ population of cells and in the (C) TRA-1-60⁺ fraction of cells. (D) Real-time PCR analysis of mRNA levels of pluripotency markers, NANOG, OCT4, SOX2 and ZFP42 in hADFs, in the cells 6 days after transducing with hSTEMCCA lentiviruses, and the cell fractions sorted by flow cytometry based on the expression of CD13, SSEA-4 and TRA-1-60 (n=2). The fold-changes were calculated relative to expression levels in hiPSCs. Data represented as mean±s.d.