Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 15;6(1):100–108. doi: 10.1242/bio.022111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 3. — Characterisation of RV-hiPSC clones isolated based RV-Tg silencing and hESC-like morphology. (A) Real time PCR analysis of pluripotency markers in the isolated RV-hiPSC clones. The fold-change was calculated relative to the expression levels in hESCs (n=2). (B) Immunofluorescence analysis of pluripotency markers in the isolated clones. (C) Real-time PCR analysis of expression of retroviral transgenes in the clones (n=2). The fold-changes were calculated relative to the expression levels in fibroblast transduced with OSKMR (Fib-OSKMR). (D) Bisulfite sequencing results of OCT4 and NANOG promoters in fibroblasts and established RV-hiPSC clones showing hypomethylation of these regions. (E) Microscopic image of an established RV-hiPSC clone confirming the stable silencing of transgenes throughout the culture. (F) In vitro differentiation of established hiPSC clones. Data from a representative clone is shown. hiPSCs formed cystic embryoid bodies (EBs) in suspension culture and these EBs were differentiated further in an adherent culture into the cells expressing markers characteristic of three germ layers – endoderm (α-feto protein, AFP), mesoderm (α-smooth muscle actin, SMA) and ectoderm (βIII-Tubulin). All images are at 10× magnification. Data represented as mean±s.d.