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. 2016 Dec 1;6(1):50–58. doi: 10.1242/bio.022772

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — The performance of the SYBR Green-based primer pairs. (A) The amplicons produced by the 9 Sybr Green primer pairs after 30 min of gel electrophoresis at 70 V. The order is as follows: ND2, ND3, ND4L, ND6, COX1, COX3, ATP1A1, GDF8, GAS7 plus 1Kb ladder, with predicted amplicons sizes of 200, 210, 150, 199, 227, 171, 170, 200 and 246 bp respectively. The discriminatory ability of ND2 (B), ND3 (C) and COX1 (D) corrected against GDF8 to estimate cross tissue mitochondrial content. All results based on SYBR Green qPCR chemistry. Results are presented as mean±s.d.