Fig. 5.
Ecdysteroid signaling regulates OA-induced Mmp2 activation. (A) A schematic showing the model of ecdysteroid signaling in the mature follicle cell to induce follicle rupture. (B–D) In situ zymography shows the reduction of gelatinase activity (green in B and C) in shd-knockdown follicles (B), which can be rescued by 20 nM 20E (C). Follicles with posterior gelatinase activity after a 6-h stimulation were separated toward the upper region in B and C and quantified in D. (E) Quantification of Oamb and mmp2 mRNA in mature follicles of control and EcRts females with qRT-PCR. ***P < 0.001.