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. 2016 Dec 19;114(4):E496–E505. doi: 10.1073/pnas.1614876114

Fig. 1.

Fig. 1.

Expression of the FGF13/miR-504 unit is up-regulated in lung cancer and is negatively regulated by p53. (A) Dot plot of FGF13 mRNA and hsa-miR-504 expression levels in lung adenocarcinoma samples from TCGA. Zero miRNA expression values were ignored. Spearman correlation and P values are indicated. (B) Box plot of FGF13 mRNA in normal and tumor samples in the TCGA lung adenocarcinoma dataset. The P value was calculated using the rank-sum test. Outliers were eliminated from box plots. n = number of samples analyzed. (C, Left) qPCR analysis of miR-504 expression normalized to small nucleolar RNA, C/D box 44 (SNORD44) in H460 cells after transient transfection with p53 siRNA (sip53) or control siRNA (siC) for 48 h. (Right) qPCR analysis of p53 mRNA; values were normalized to GAPDH. Data are expressed as means ± SD from three independent experiments. *P < 0.05. (D, Upper) FGF13 and p53 mRNA expression, normalized to GAPDH, of cells treated as in C. Data are expressed as means ± SD from three independent experiments. ***P < 0.001. (Lower) Cell lysates from the same experiment were subjected to Western blot analysis with the indicated antibodies. GAPDH served as loading control. (E, Upper) FGF13 and p53 mRNA expression, normalized to GAPDH, 48 h after transient transfection of H460 cells with siRNAs targeting p53 (sip53), FGF13 (siFGF13), control siRNA (siC), or combinations thereof. Data are expressed as means ± SD of duplicates from a representative of three independent experiments. (Lower) Western blot analysis of the same experiment with antibodies against FGF13 and GAPDH (loading control).