Table S1.
Analysis of IFN protein from HIEs
| IFN sample analyzed | 6 hpi | 8 hpi | 12 hpi | 20 hpi | 24 hpi | 48 hpi |
| IFN-λ1 | ||||||
| Supernatant | − | − | − | − | − | |
| Concentrated supernatant | − | − | ||||
| Supernatant + anti-IL28RA | − | − | − | |||
| Cells + supernatant | − | − | ||||
| IFN-λ2/3 | ||||||
| Supernatant | − | − | ||||
| Supernatant + anti-IL28RA | − | − | ||||
| Cells + supernatant | − | − | ||||
| IFN-β1 | ||||||
| Supernatant | − | − | −* | − | − |
MOI > 10 FFU per cell for each experiment. −, below the limit of detection. “Concentrated supernatant” indicates supernatant from n = 5–6 samples concentrated into a single sample. “Supernatant + anti-IL28RA” indicates supernatant from HIEs incubated with anti-IL28RA antibody (25 μg/mL) during infection to block the type III IFN receptor. “Cells + supernatant” indicates sonicated homogenate of HIEs and surrounding media. IFN-λ1 ELISA sensitivity is 8 pg/mL. IFN-λ2/3 ELISA sensitivity is 15.6 pg/mL. and IFN-β1 ELISA sensitivity is 50 pg/mL.
*
Limit of detection is 2 pg/mL.