Table S7.
The effect of dietary nitrate on leukocyte surface expression of CD11b, CD62L, and CD162
| CD11b, CD62L, and CD162 | Chow | Western | ||||
| KCL | KNO3 | P value | KCL | KNO3 | P value | |
| CD11b | ||||||
| Neutrophil; Gr1+ | 2,876 ± 357 | 2,076 ± 169* | 0.04 | 2,471 ± 137 | 2,973 ± 213 | 0.06 |
| Resident monocyte; F4/80+ | 7,821 ± 606 | 6,577 ± 423 | 0.10 | 6,038 ± 289 | 6,751 ± 534 | 0.24 |
| Inflammatory monocyte; F4/80+Gr1+ | 8,927 ± 872 | 7,735 ± 452 | 0.21 | 8,198 ± 357 | 9,658 ± 541* | 0.03 |
| T-cell CD4+ | 3,868 ± 1,672 | 3,476 ± 987 | 0.53 | 4,513 ± 1,430 | 3,963 ± 1,167 | 0.18 |
| T-cell CD8+ | 3,036 ± 1,611 | 2,980 ± 1,084 | 0.93 | 3,302 ± 1,210 | 3,339 ± 883 | 0.93 |
| B-cell CD19+ | 1,552 ± 651 | 1,558 ± 542 | 0.98 | 1285 ± 497 | 1,352 ± 393 | 0.72 |
| Percent neutrophil; Gr1+-expressing | 99.7 ± 0.1 | 99.6 ± 0.1 | 0.25 | 99.8 ± 0.1 | 99.9 ± 0.0 | 0.08 |
| Percent resident monocyte; F4/80+-expressing | 96.0 ± 0.8 | 95.2 ± 1.2 | 0.61 | 92.6 ± 1.7 | 91.2 ± 1.7 | 0.56 |
| Percent inflammatory monocyte; F4/80+Gr1+-expressing | 100 ± 0.0 | 99.9 ± 0.1 | 0.16 | 99.8 ± 0.1 | 99.7 ± 0.1 | 0.76 |
| Percent CD4+ cells-expressing | 12.6 ± 7.5 | 16.2 ± 3.1 | 0.31 | 16.7 ± 7.2 | 15.1 ± 4.6 | 0.53 |
| Percent CD8+ cells-expressing | 17.5 ± 9.2 | 25.6 ± 5.2* | 0.03 | 28.2 ± 10.4 | 26.6 ± 8.6 | 0.68 |
| Percent CD19+ cells-expressing | 4.4 ± 4.1 | 3.3 ± 1.5 | 0.4 | 3.9 ± 1.4 | 6.3 ± 7.2 | 0.28 |
| CD62L | ||||||
| Neutrophil; Gr1+ | 5,060 ± 351 | 5,155 ± 249 | 0.82 | 4,516 ± 267 | 4,581 ± 302 | 0.87 |
| Resident monocyte; F4/80+ | 2,315 ± 265 | 2,528 ± 295.7 | 0.60 | 2,459 ± 203 | 3,002 ± 501 | 0.32 |
| Inflammatory monocyte; F4/80+Gr1+ | 3,045 ± 244 | 3,224 ± 197 | 0.57 | 4,093 ± 501 | 4,120 ± 374 | 0.97 |
| T-cell CD4+ | 2,562 ± 416 | 2,655 ± 336 | 0.59 | 3,070 ± 194 | 3,138 ± 587 | 0.7 |
| T-cell CD8+ | 3,501 ± 607 | 3,660 ± 620 | 0.57 | 3,947 ± 443 | 3,947 ± 720 | 1 |
| B-cell CD19+ | 2,003 ± 380 | 1,945 ± 259 | 0.69 | 2,484 ± 497 | 2,434 ± 479 | 0.8 |
| Percent neutrophil; Gr1+-expressing | 99.6 ± 0.1 | 99.7 ± 0.1 | 0.54 | 99.4 ± 0.1 | 99.7 ± 0.1* | 0.02 |
| Percent resident monocyte; F4/80+-expressing | 19.0 ± 2.9 | 18.9 ± 7.8 | 0.97 | 31.8 ± 4.1 | 37.2 ± 4.7 | 0.40 |
| Percent inflammatory monocyte; F4/80+Gr1+-expressing | 89.2 ± 1.6 | 89.8 ± 1.7 | 0.8 | 91.7 ± 1.2 | 94.3 ± 1.1 | 0.12 |
| Percent CD4+ cells-expressing | 78.2 ± 7.6 | 76.5 ± 12.1 | 0.7 | 72.2 ± 6.6 | 74.5 ± 8.3 | 0.47 |
| Percent CD8+ cells-expressing | 92.4 ± 4.3 | 87.6 ± 8.4 | 0.13 | 88.6 ± 4.4 | 88.2 ± 5.8 | 0.83 |
| Percent CD19+ cells-expressing | 92.0 ± 4.4 | 92.2 ± 2.8 | 0.92 | 92.7 ± 3.5 | 94.2 ± 1.0 | 0.17 |
| CD162 | ||||||
| Neutrophil; Gr1+ | 22,631 ± 903 | 23,345 ± 1099 | 0.64 | 21,027 ± 712 | 20,414 ± 1,252 | 0.67 |
| Resident monocyte; F4/80+ | 14,147 ± 1,086 | 12,869 ± 379 | 0.23 | 14,867 ± 1,476 | 11,743 ± 557 | 0.07 |
| Inflammatory monocyte; F4/80+Gr1+ | 29,424 ± 1,932 | 30,559 ± 1,706 | 0.67 | 34,023 ± 1,265 | 32,211 ± 1,355 | 0.34 |
| T-cell CD4+ | 8,496 ± 642 | 8,708 ± 1,249 | 0.67 | 8,734 ± 1,047 | 8,425 ± 1,245 | 0.53 |
| T-cell CD8+ | 13,577 ± 2,013 | 15,926 ± 3,439 | 0.2 | 16,597 ± 4,052 | 16,318 ± 3,355 | 0.59 |
| B-cell CD19+ | 457 ± 146 | 494 ± 240 | 0.71 | 557 ± 241 | 685 ± 626 | 0.52 |
| Percent neutrophil; Gr1+-expressing | 100 ± 0 | 100 ± 0 | 0.34 | 100 ± 0 | 99.9 ± 0.1 | 0.27 |
| Percent resident monocyte; F4/80+-expressing | 97.3 ± 2.3 | 92.9 ± 5.8 | 0.54 | 90.9 ± 5.3 | 81.9 ± 7.2 | 0.32 |
| Percent inflammatory monocyte; F4/80+Gr1+-expressing | 99.6 ± 0.3 | 99.8 ± 0.1 | 0.5 | 99.9 ± 0.1 | 99.9 ± 0.1 | 0.86 |
| Percent CD4+ cells-expressing | 100 ± 0 | 100 ± 0 | 1 | 99.9 ± 0.1 | 100 ± 0.1 | 0.32 |
| PercentCD8+ cells-expressing | 100 ± 0 | 100 ± 0 | 1 | 100 ± 0 | 100 ± 0 | 1 |
| Percent CD19+ cells-expressing | 83.8 ± 4.1 | 82.9 ± 6.0 | 0.75 | 76.1 ± 15.8 | 79.9 ± 11.6 | 0.51 |
The effect of dietary nitrate on circulating neutrophils, resident monocytes, inflammatory monocytes, and T and B cells of CD11b, CD162, and CD62L expression, as measured by MFI, and the percentage of cells expressing these markers in ApoE KO mice. Data shown as mean ± SEM of n = 12–15 for myeloid cells and n = 10–12 for lymphoid cells. Statistical significance was determined using unpaired t test represented by *P < 0.05 compared with KCl treatment in each diet.