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. 2017 Jan 30;7:41597. doi: 10.1038/srep41597

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Multipotent neural stem cells NS-5 give rise to astrocytes, neurons and oligodendrocytes. Cell type-specific markers GFAP (astrocytes), βIII-tubulin (neurons) and O4 (oligodendrocytes) were analyzed. (B) Quantitative RT-PCR analysis of Disp3 mRNA in undifferentiated and differentiated cells. Ubb was used as a reference gene. Bars represent the mean of three independent samples with error bars indicating standard deviation and the level of statistical significance (**P < 0.01, ***P < 0.001). Mean values: undifferentiated cells = 1.00, astrocytes = 0.45, neurons and oligodendrocytes = 0.75. (C) Immunofluorescence images of undifferentiated and differentiated cells stained with a polyclonal DISP3 antibody (green) and cell type-specific markers (red). DAPI (blue) was used to stain the nuclei.