Table 1.
Features summary of elF5A mutants. ND, not determined; TS, temperature sensitive; TR, temperature resistant. + indicates levels of elF5A protein.
| Source | Mutation | Growth phenotypea |
Expression of elF5A |
elF5A protein levels at the restrictive temperature |
|---|---|---|---|---|
| TIF51A | ____ | TR | Yes | ++++ |
| tif51A(K51R) [1] | K51R | Nonviable | Yes | ND |
| ts 1159 [26] | S149P | TS 34 °C | Yes | + |
| tif51A-1 [27] | P83S | TS 36 °C | Yes | + |
| tif51A-2 [27] | P83L | TS 37 °C | Yes | +++ |
| tif51A-3 [27] | C39Y/G118D | TS 36 °C | Yes | ++ |
| C39Y | TS 38 °C | Yes | +++ | |
| G118D | TR | Yes | ND | |
| Synthetic lethality | C39Y/P83L/G118D | Nonviable | ND | ND |
| Synthetic lethality | C39Y/T66I/G118D | Nonviable | ND | ND |
| T66I/G118D | TS 37 °C | Yes | +++ | |
| T66I | TS 38 °C | ND | +++ | |
| Synthetic lethality | C39Y/G118D/S140F | Nonviable | ND | ND |
| G118D/S140F | TS 38 °C | Yes | + | |
| S140F | TR | Yes | ND | |
| Synthetic lethality | C39Y/G118D/M142I | Nonviable | ND | ND |
| G118D/M142I | TS 38 °C | Yes | ++ | |
| M142I | TR | Yes | ND | |
| Synthetic lethality | C39Y/P116L/G118D | Nonviable | ND | ND |
| P116L/G118D | TS 38 °C | Yes | ++ | |
| Synthetic lethality | C39Y/G50D/G118D | Nonviable | ND | ND |
| C39Y/G50D | Nonviable | Yes | ND | |
| G50D | Nonviable | Yes | ND | |
| Synthetic lethality | C39Y/G53D/G118D | Nonviable | ND | ND |
| C39Y/G53D | Nonviable | Yes | ND | |
| G53D | Nonviable | Yes | ND | |
| PCR mutagenesis | N29K/M44I/P83S | TS 37 °C | Yes | ++ |
| M44I/P83S | TS 37 °C | Yes | ++ | |
| N29K | TR | Yes | ND | |
| M44I | TR | Yes | ND | |
| PCR mutagenesis | G14D/V57D | TS 37 °C | Yes | +++ |
| G14D | TR | Yes | ND | |
| V57D | TS 37 °C | Yes | +++ | |
| PCR mutagenesis | Q22H/L93F | TS 38 °C | Yes | ++++ |
| L93F | TR | Yes | ND | |
| Q22H | TR | Yes | ND | |
| Site-directed mutagenesis | G50A | Nonviable | Yes | ND |
| Site-directed mutagenesis | H52A | Nonviable | Yes | ND |
| Site-directed mutagenesis | H52D | Nonviable | Yes | ND |
| Site-directed mutagenesis | G53A | Nonviable | Yes | ND |
| Site-directed mutagenesis | H54D | Nonviable | Yes | ND |
| Site-directed mutagenesis | K56D | Nonviable | Yes | ND |
| Site-directed mutagenesis | K56A | TS 37 °C | Yes | ++++ |
| Site-directed mutagenesis | Δ1–10 | TR | Yes | ND |
| Site-directed mutagenesis | Δ116–157 | Nonviable | No | ND |
| Site-directed mutagenesis | S24P | TS 37 °C | Yes | ++ |
| Site-directed mutagenesis | G118V/D122W | TS 37 °C | Yes | ++ |
| Site-directed mutagenesis | G118D/L120N/L124D | Nonviable | No | ND |
a
The indicated temperatures are the lowest temperature at which the phenotype is observed.