Figure 3. The FACS analysis indicates that CG200745 induces apoptosis of pancreatic cancer-cell lines.
The doses of erlotinib and CG200745 were equivalent to IC 20~30. The apoptosis rate of BxPC3 cells cultured 72 h after drug administration in the (A) control, (B) 0.01 μM gemcitabine, (C) 0.01 μM gemcitabine + 0.288 μM CG200745, (D) 0.01 μM gemcitabine + 0.015 μM erlotinib, (E) 0.01 μM gemcitabine + 0.288 μM CG200745 + 0.015 μM erlotinib. Annexin V-FITC/PI double staining; Q1, autophagic cell death; Q2, late apoptotic cells; Q3, normal cells; Q4, early apoptotic cells.