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. 2017 Jan 30;7:41616. doi: 10.1038/srep41616

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(a) Neferine enhanced OXA-induced apoptosis of HCC cells (OXA vs. OXA + Neferine, p < 0.001, respectively). Apoptosis rate in HCC treated with 10 μM Neferine and/or 5 μM OXA for 48 hrs were detected with flow cytometry by Annexin V/PI staining. (b) 10 μM Neferine downregulated anti-apoptotic protein of Bcl-2, but upregulated apoptotic molecular of caspase 3 and Bax in 5 μM OXA-treated HCC cells for 48 hrs. ^***p < 0.001.