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. 2017 Jan 30;7:41616. doi: 10.1038/srep41616

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(a,b) Migration and invasion abilities suppressed by Neferine treatment were restored by Snail overexpression. HepG2 and Bel-7402 cells were pre-treated with or without 10 μM Neferine and then transfected by pcDNA3.1-Snail and pcDNA3.1-NC vectors for 48 hrs, respectively. #: pcDNA3.1-NC + Nef vs. pcDNA3.1-NC, p < 0.05, respectively; + : pcDNA3.1-Snail vs. pcDNA3.1-NC, p < 0.01, respectively; Δ: pcDNA3.1-Snail + Nef vs. pcDNA3.1-Snail, p < 0.01, respectively. (c) OXA sensitization by Neferine treatment was eliminated by Snail overexpression in HepG2 and Bel-7402 cells. (d) OXA sensitization by Neferine treatment was eliminated by 10 ng/ml TGF-β1 in HepG2 and Bel-7402 cells. #: Nef + OXA vs. OXA, p < 0.01, respectively; +: Nef + OXA + TGF-β1 vs. Nef + OXA, p < 0.001, respectively. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.