Figure 4. VLDL oxidation.

A, VLDL from 3ko and 4ko mice (1 μg) was incubated with 10 μm Cu²⁺. The ordinate shows lag time (average of three experiments) until the inflection point on 234 nm absorption curves. B, TBARS after Cu²⁺ oxidation of VLDL isolated from 3ko and 4ko mice. TBARS were measured after 16 h of incubation with 10 μm Cu²⁺ at 0.5 mg protein/ml. C, TBARS in the medium of MPM incubated with VLDL in culture. Thioglycolate-elicited MPM isolated from either 4ko or 3ko mice was incubated with 50 μg/ml apoE-deficient VLDL. TBARS in medium were measured after 24 h. *, p ≤ 0.05; **, p ≤ 0.005; ***, p ≤ 0.0005 with Tukey-Kramer HSD.