Figure EV2. Defective forms of IL‐2Rα protein are stabilized by MG‐132.

• A, B
  Mislocalized IL‐2Rα protein was stabilized by MG‐132. Anti‐Flag immunosignals with (+) or without (−) MG‐132 were quantified at the indicated time points. The data represent mean ± SD calculated from three independent experiments (n = 3). Statistical analysis by Welch's t‐test (*P < 0.01; N.S., non‐significant).
• C, D
  Anti‐Flag immunosignals of IL‐2Rα ΔSS and IL‐2Rα ΔSSΔTM (C) as well as those of low‐mobility (indicated as glycosylated) and high‐mobility (indicated as non‐glycosylated) signals of IL‐2Rα WT (D) (shown in Fig 3G as a representative example) were quantified. The data represent mean ± SD calculated from four independent experiments (n = 4). Statistical analysis by Welch's t‐test (*P < 0.05; N.S., non‐significant).

Source data are available online for this figure.