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. 2016 Apr 22;17(6):842–857. doi: 10.15252/embr.201541402

Figure 5. UBQLN4 recognizes misassembled IL‐2Rα through its unembedded TMD .

Figure 5

  1. A series of Flag‐tagged truncated mutants of IL‐2Rα substrates were expressed in HeLa cells with T7‐tagged UBQLN4 and treated with (+) or without (−) 10 μM MG‐132 for 4 h. Flag‐IL‐2Rα substrates were immunoprecipitated, and their co‐precipitation with UBQLN4 was analyzed.
  2. T7‐UBQLN4 was immunoprecipitated, and its interactions with a series of IL‐2Rα proteins were detected.

Source data are available online for this figure.