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. 2017 Jan 1;144(1):63–73. doi: 10.1242/dev.138545

Fig. 6.

Fig. 6.

The increased pool size of the pentose phosphate pathway (PPP) during CB. (A) PPP metabolites were quantified by IC-MS. Area values were normalized to the amount of protein extracted from the embryos, and the relative ratios to E8.5 embryos are shown. n=4 independent sample replicates. *P<0.05, **P<0.01 versus E8.5 (two-tailed Welch's t-test). E8.5, ss 8-11; E9.5, ss 24-25; E10.5, ss 34-35. G-6-P, glucose 6-phosphate; F-6-P, fructose 6-phosphate; F-1,6-P2, fructose 1,6-bisphosphate; DHAP, dihydroxyacetone phosphate; GA-3-P, glyceraldehyde 3-phosphate; 6-PG, 6-phosphogluconate; Ru-5-P, ribulose 5-phosphate; R-5-P, ribose 5-phosphate; X-5-P, xylulose 5-phosphate; S-7-P, sedoheptulose 7-phosphate; E-4-P, erythrose 4-phosphate. (B,C) Amounts of glycerol 3-phosphate and glycine determined by CE-MS were normalized to the total amount of protein extracted from the embryos, and the relative ratios to E8.5 embryos are shown. n=3 independent sample replicates. *P<0.05 versus E8.5 embryos (two-tailed Welch's t-test). E8.5, ss 10-12; E9.5, ss 24-26; E10.5, ss 35-37. Data are represented as mean±s.d.