Figure 3. P-gp, BCRP and Mrp 2 protein expression in brain and spinal cord capillaries of SOD rats at ALS pre-onset, onset and symptomatic stage and age-matched wildtype rats.
Representative westernblots (top) and densitometric analysis (bottom) of transporter expression in 10 µg of plasma membrane lysates of brain and spinal cord capillaries. Four rat brains and spinal cords (pooled samples from two to three capillary preparations) were utilized in each SOD group and their respective wildtype (WT) groups. Data represent the percent change in transporter expression first normalized to actin expression and then normalized to age-matched wildtypes.