Fig. 6.

atl loss triggers increased ROS generation and increased JNK and Foxo activity in muscle. All animals analyzed were males reared as mating pairs. (A–D) Live imaging of dissected thoracic tissue from elav>dcr2 (A) and elav>dcr2,atlRNAi (B), w¹¹¹⁸ (C) and atl² (D) adults immediately following eclosion (day 0) and treated with the ROS indicator dihydroethidium (DHE). DFM49 has been imaged. Scale bars: 25 µm. (A–D) Maximum intensity z-projections from a confocal z-stack. (E) Quantification of ethidium pixel intensity per µm³ of DFM49 in elav>Dcr2 (blue circles represent each individual data point), elav>Dcr2,atlRNAi (green circles represent each individual animal DFM49), w¹¹¹⁸ (red circles represent each individual animal DFM49) and atl² (cyan circles represent each individual animal DFM49). Means±s.e.m. are indicated. P=0.035 for elav>dcr2 versus elav>dcr2,atlRNAi, and P=0.004 for w¹¹¹⁸ versus atl². (F–I) Dissected thoracic tissue from elav>dcr2 (F), elav>dcr2,atlRNAi (G), w¹¹¹⁸ (H) and atl² (I) adults, each also expressing puc-lacZ, at 3 days following eclosion (F,G) or immediately following eclosion (H,I) and treated with an antibody against β-galactosidase. Scale bar: 25 µm. The images are maximum intensity z-projections from a confocal z-stack. (J–M) Dissected thoracic tissue from elav>dcr2 (J), elav>dcr2,atlRNAi (K), w¹¹¹⁸ (L) and atl² (M) adults, each also expressing 4E-BP-lacZ, immediately following eclosion and treated with an antibody against β-galactosidase. Scale bars: 25 µm. Images are maximum intensity z-projections from a confocal z-stack. An image that is representative of three animals is shown for each condition.