Intracellular staining of ALNCs isolated from mice epicutaneously (EC) immunized with PBS, OVA, OVA and CpG, or CpG alone for 2 weeks before FACS analysis. Absolute numbers of TCRβ+CD4+IFN-γ+
(A), TCRβ+CD8+IFN-γ+
(B), TCRγδ+IFN-γ+
(C), TCRβ+CD4+IL-17A+
(D), TCRβ+CD8+IL-17A+
(E) cells are shown. Results are expressed as means ± SEs (n = 4–6). ns, Not significant. *P < .05, **P < .01, and ***P < .001.