FIG 1.

Characterization of pyrazinoic acid (POA)-resistant panD wild-type M. tuberculosis strains. Growth inhibition dose-response curves of 9 POA-resistant panD wild-type strains, POA^r 11 to 19, POA-sensitive wild-type M. tuberculosis H37Rv, and a representative POA-resistant panD mutant strain, POA^r 1, isolated previously (14), for (A) POA, (B) rifampin (RIF), and (C) isoniazid (INH). Experiments were carried out 3 times independently with technical replicates. Mean values and standard deviations from results of representative experiments are shown. (D) Location of 7 ClpC1 amino acid sequence polymorphisms in POA-resistant panD wild-type M. tuberculosis strains POA^r 12 to 19. ClpC1 domain organization is shown as described in reference 23. Within the N-terminal domain, two repeats are labeled I and II. A and B in the D1 and D2 domains indicate Walker A and Walker B motifs, respectively. (E) Location of the nucleotide sequence polymorphism G to C (−10) in the untranslated leader mRNA of clpC1 in POA-resistant panD wild-type M. tuberculosis strain POA^r 11. The organization of the clpC1 upstream region is shown as described in reference 20. A conserved TANNNT promoter motif (TACAGT) and the transcriptional start site (TSS), located 55 bp upstream of the clpC1 coding sequence, are indicated (20). Refer to Table 1 for genotypes and phenotypes of strains.