FIG 4.

Downregulation of miR-21 in LX2 cells via the lentiviral vector. The lentiviral vector interfered with LX2 cells for 72 h to mediate miR-21 down expression. (A) The expression of GFP was observed with a fluorescence microscope after lentivirus was introduced into LX2 cells for 48 and 72 h. (B) Expression of miR-21, Smad7, and CTGF was detected by quantitative real-time PCR. (C) Expression of protein was assayed by Western blotting. The data are shown as means and SEM; n = 3. *, P < 0.01 for lentivirus-down versus control groups, a significant difference from the respective values determined by ANOVA.