TABLE 1.
Primer sequences for real-time quantitative PCR
| Gene | Primer | Sequence (5′→3′) |
|
|---|---|---|---|
| Human | Mouse | ||
| miR-21 | RT stem-loop | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCAACATC | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAACATC |
| Forward | ACACTCCAGCTGGGTAGCTTATCAGACTGA | ATGGTTCGTGGGTAGCTTATCAGACTGA | |
| Reverse | TGGTGTCGTGGAGTCG | GCAGGGTCCGAGGTATTC | |
| Smad7 | Forward | AGAGGCTGTGTTGCTGTGAAT | GTGTTGCTGTGAATCTTACG |
| Reverse | GCAGAGTCGGCTAAGGTGATG | AGAAGAAGTTGGGAATCTGA | |
| CTGF | Forward | AATGCTGCGAGGAGTGGGT | TCAACCTCAGACACTGGTTTCG |
| Reverse | CGGCTCTAATCATAGTTGGGTCT | TAGAGCAGGTCTGTCTGCAAGC | |
| GAPDH | Forward | ACCACAGTCCATGCCATCAC | ACCACAGTCCATGCCATCAC |
| Reverse | TCCACCACCCTGTTGCTGTA | TCCACCACCCTGTTGCTGTA | |