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Increased binding of BODIPY-labeled daptomycin (BDP-DAP) by tazobactam (TZ) or SLB for MRSA Sanger 252 (A) or hVISA D592 (B). Cells were incubated for 20 min with BODIPY-DAP at 8 mg/liter (Sanger 252) or 16 mg/liter (D592) after growth to an OD600 of 0.5 in antibiotic-free medium (control) or with TAZ or SLB. Error bars show standard errors of the means for 3 analyzed fields. *, P < 0.001 (A) or P < 0.05 (B); **, P < 0.0001 (A).
