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Histogram from a flow cytometry experiment using compensation beads stained with either 0.5 μg of chlorin–antibody FbC2–Ab (solid line) or 0.12 μg of Brilliant Violet 650–antibody BV650–Ab (dashed line), plus unstained beads (dotted line). The coefficient of variation (CV) was 36 (FbC2–Ab), 30 (BV650–Ab), and 155 (unstained beads). The analysis used the 405 nm violet laser with 600 nm longpass and 620/60 nm bandpass filters.
